Spectrophotometric determination of the pKa of Bromothymol Blue. Solutions of different concentration of the indicator were used. MDL number MFCD00077263. 1 decade ago. Ensure that the solutions are mixed well. Given the equation: y= 48048x- 0.0105, find the molar absorptivity and use this value in your experiment. ,IC10113005EA,IC10113025EA. Beilstein/REAXYS Number 6887926 . EC Number 252-169-7. The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. Bromothymol blue, Powder ACS. In this experiment, spectrophotometry is employed to measure the pKa of bromothymol blue, an acid-base indicator. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. Other indicators work in different pH ranges. Supplier: MP Biomedicals. Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. Assume a cuvet with a 14.45 mm diameter was used. They would both appearyellow. There is a linear relationship between log ( [ln-]/ [Hln]) and pH. NACRES NA.47 Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. Bromothymol blue is an acidic pH chromoionophore where acid to base change corresponds to its neutral to anionic form, respectively. PubChem Substance ID 24892083. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). The pKa can be determined from the spectrophotometric data using different methods. Molecular Weight 646.36 . 2 Answers. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . Answer Save. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. Favorite Answer. Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. Molar absorptivity ___ (You will be using this value in your experiment.) 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